November 26, 2019 by admin 0 Comments

The 2019 ASEAN-Republic of KOREA Commemorative Summit – Rokit Healthcare

“로킷헬스케어, 한·아세안 정상회담 부대행사 K뷰티 페스티벌 참여”– ROKIT Healthcare

  • 중기부, 복지부 주관 ‘K뷰티 페스티벌’ 참여… 화장품 독성시험 인공피부 플랫폼 선보여

  • “토탈헤어 플랫폼 등 다양한 첨단기술 아세안 국가에 전파 할 것”

춤형 재생의학 전문기업 로킷헬스케어(대표이사 유석환)가 부산 벡스코에서 열리는 ‘한·아세안 K뷰티 페스티벌’ 참여했다. ‘한·아세안 특별 정상회의’ 부대 행사인 ‘K뷰티 페스티벌’은 오늘까지 진행된다.

▲로킷헬스케어가 부산 벡스코에서 열리는 ‘한아세안 K뷰티 페스티벌’에 참가해 전시부스를 운영하고 있다.

이번 한·아세안 K뷰티 페스티벌 (ASEAN-ROK K-Beauty Festival)은 ‘아세안의 아름다움, 함께 이루는 꿈(ASEAN Beauty, Make up Our Dreams)’이라는 슬로건 아래 보건복지부, 식약처 등 관련 부처 및 정부유관기업과 다수의 뷰티 기업들이 참여한 전시회다.

국내 다수의 화장품 기업 중 로킷헬스케어는 보건복지부의 지원을 받아 K뷰티 체험존을 운영하고 제품을 전시하고 있다. 이밖에도 자사의 ▲바이오프린터인 Dr. INVIVO 4D 및 Dr. INVIVO 4D6 ▲화장품 독성시험 인공피부 플랫폼 EpiTem ▲토탈 헤어 플랫폼 아쿠아드폴리 ▲안티에이징 건강기능식품인 NMN ▲다이어트 보조제 하이비키니를 선보이고 있다. 한 아세안 정상의 영부인들도 로킷헬스케어의 부스에 방문하여 인공피부 플랫폼에 관심을 보였다.

November 15, 2019 by admin 0 Comments

Development of an effective sample transfer device for biomarker detection in nasal secretions

Authors
Young Ju Lee (a), Jae-Chul Lee (b), Young Gyu Eun (c), Gi-Ja Lee (a)
Abstract
Nasal secretions (NS) reflect inflammatory activity of the nasal mucosa and thus can be utilized for disease diagnosis and determining treatment effects in Allergic rhinitis (AR). However, non-standardized collection of samples can affect the measured concentration of inflammatory biomarker in NS. In this study, we aimed to develop and evaluate new devices capable of standardizing the collection, storage, and preprocessing methods of NS samples. First, we chose the best swab as polyester (PE) and selected a stimulation method, twirling for 10 s at 1 Hz, to efficiently release AR biomarkers from a PE swab. Storage of sample solutions at −20 °C was optimal for the stability of biomarkers for the detection of AR. The new swab sample transfer device showed excellent concentration recovery efficiency (90–100%) for tryptase (Trp) and eosinophil cationic protein (ECP) without crosstalk between the two biomarkers. Finally, we compared the concentration of Trp in human NS samples of AR patients (n = 6) pre-processed by the new device with that by centrifuge as a standard method. As a result, the concentrations of Trp in NS were very similar in both groups. Therefore, this device can be utilized as an effective sample transfer and pre-processing device for point-of-care testing of AR.

November 1, 2019 by admin 0 Comments

Coaxial bioprinting of cell-laden vascular constructs using a gelatin–tyramine bioink

Authors
Soyoung Hong (a), Ji Seon Kim (a), Boyoung Jung (a), Chonghyun Won *(b), Changmo Hwang *(a, c)
Abstract
Herein, three-dimensional (3D) bioprinting of engineered constructs with cell-laden biomaterials was investigated for the development of 3D tissue constructs in vitro. The present article proposes a simple coaxial-nozzle-based printing method using a one-step gelling gelatin bioink containing different cell types for vascular structure generation. First, a gelatin bioink prepolymer with a tyramine functional group was synthesized. To facilitate rapid gelation, polyethylene glycol (PEG) was introduced as a spacer between gelatin and tyramine. The gelatin–PEG–tyramine (GPT) prepolymer underwent enzymatic crosslinking, which yielded a higher gelation rate of up to 4.24 ± 0.08 s. Second, one-step bioprinting of a cell-laden tubular structure was demonstrated using a coaxial type extruder and the GPT bioink with human umbilical vein endothelial cells (HUVECs) with or without human dermal fibroblasts (HDFs). The printed no-cell GPT tube was demonstrated to possess a perfusable vascular structure. The extruded tube with HUVECs-in-GPT sheath configuration resulted in an endothelial cell-lined hollow structure and was maintained for up to 8 days in vitro. Additionally, the coaxially extruded tube with HUVECs-in-core (gelatin) and HDFs-in-GPT sheath (GPT) configuration exhibited a distribution of these two cell types along the tube axis. In the current study, it was demonstrated that a radial distribution of multiple vascular cells can be simply achieved using a synthetic GPT bioink combined with a coaxial nozzle printing system, serving as a proof-of-concept for one-step generation of vascular constructs. The rapid gelling bioink prepolymer, in combination with a coaxial bioprinter nozzle mechanism, has great potential for the development of designed, printed, and organized 3D tissue architecture vascularization.