Based on computer-aided motion, extrusion-based 4D bioprinting precisely dispenses living cells and biomaterials layer by layer, generating structures that imitate native human tissues and biological systems. Because these are often further incubated to proliferate, mature and change over time, this dimension of time makes it 4D. The technology brings key values of automation, biomimetic architecture, and on-demand biomanufacturing. It is widely applicable to the fields of bioengineering and medicine, from in vitro applications in novel product screening, disease modeling, and animal alternative experimentation to in vivo applications in tissue transplantation and regeneration.
About 4D Bioprinting
What is 4D Bioprinting?
In-situ 4D Printing
Vessel Printing
Tissue Engineering
About Dr. INVIVO 4D2
What is Dr. INVIVO 4D2?
Dr. INVIVO 4D2 is the first clean-chamber bioprinter pioneering the way for effective, on-demand biofabrication of human tissue models and implants.
It is capable of printing freeform cell suspensions, hydrogels, thermoplastic filaments, pastes, and other composite materials, enabling both hard and soft tissue engineering.
First Clean-Chamber Bioprinter
HEPA filter, UV lamps for disinfection, chamber temperature display and HD camera
Standard
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Dr. INVIVO 4D2
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Temperature Controller
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Air Dispenser Controller
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Air Compressor
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Upgrade
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Dr. INVIVO 4D2
O
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Temperature Controller
O
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Air Dispenser Controller
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Air Compressor
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Premium
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Dr. INVIVO 4D2
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Temperature Controller
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Air Dispenser Controller
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Air Compressor
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Maximum Freedom of Design
• Highest temperature and extrusion pressure range:
• -4℃ ~ 350℃ and up to 980 kPa
• Able to use a wide range of biomaterials:
• including cell suspensions, hydrogels, thermoplastic filaments,
• pellets and pastes necessary for soft and hard tissue bio-fabrication
Syringe Dispenser
Nozzle size:
100µm ~ 600µm
100µm ~ 600µm
Low viscosity materials printing:
3 – 300 kPa pressure
(0.03 – 3 bar)
3 – 300 kPa pressure
(0.03 – 3 bar)
Operating Temperature Range:
-4 ~ 60°C
-4 ~ 60°C
Collagen, Hyaluronic Acid, Gelatin,
Pluronic Acid, Chitosan, etc.
Pluronic Acid, Chitosan, etc.
Fillament Extruder
Nozzle size:
100µm~ 400µm
100µm~ 400µm
Operating Temperature Range:
RT ~ 250℃
RT ~ 250℃
PCL, PLA, PLGA, etc. Filaments
Hot-melt Pneumatic
Dispenser
Nozzle size:
100µm ~ 600µm
100µm ~ 600µm
High viscosity materials printing:
970 kPa pressure (9.7 bar)
970 kPa pressure (9.7 bar)
Operating Temperature Range:
RT ~ 350℃
RT ~ 350℃
PCL, PLA, PLGA, Polyurethane etc.
Temperature control and Pressure control
In its entirety, Dr. INVIVO 4D2 supports printing temperature from below zero up to 350 ℃ and a pressure up to 9.7 bar with dedicated temperature control and air compressor powerhouses provided in addition to the main printer unit.
-4℃
Bed(-4℃~60℃)
60℃
Syringe(-4℃~60℃)
250℃
Filament(RT~250℃)
350℃
Hot-melting(RT~350℃)
Saving Lives
Dr. INVIVO 4D2 is paving the way in translational research of the bioprinting technolgy for treating chronic conditions such as diabetic foot ulcers and osteoarthritis.
• Skin tissue regeneration for diabetic foot ulcer patients
• Cartilage regeneration for arthritis patients
Applications
Dr. INVIVO 4D2 finds applications in
1. Tissue Engineering and Regenerative Medicine
Produce 3D structures consisting of living cells, biomaterials, and active biomolecules to mimic human tissues for in vitro studies and novel regenerative approaches
2. Toxicology Testing
Use engineered 3D tissues for testing chemical toxicity and efficacy of novel compounds, including cosmetic products and drugs
3. Modeling of Cancer Microenvironments
Recreate complex tumor microenvironments by integrating immune cells, vasculature and stromal cells with tumor spheroids through the printing of compartments and channels within hydrogel matrices
4. Drug Printing and Discovery
Fabricate on-demand fully customizable tablets with personalized shape, porosity, and concentration for drug-loading and release control
5. Soft Robotics
Integrate soft, biocompatible materials with traditional robotics for enhancing function, chemical controls, bio-triggered contraction, and self-healing capabilities upon damage with applications in biosensors and devices
Relevant Publications
How to Use?
ROKIT Offerings
User References
