A cell culture biomaterial supplement consisting
of full native human ECM (Extracellular Matrix),
useful for in vitro 4D bioprinting
and cell culturing as well as clinical translational studies

HumaTein™
about ECM
What is ECM?

ECM (Extracellular Matrix) is a protein complex, consisting of more than 300 proteins, peptides, and polysaccharides. ECM supports cell attachment, proliferation and migration, and it roles as a growth factor reservoir.

about HumaTein™
What is HumaTein™?

HumaTein™ is a human cell derived whole Extracellular Matrix(ECM), comprised of more than 300 proteins and associated cytokines.
HumaTein™ opens new possibilities for regeneration researches and relative studies.

Available at

ROKIT products are available to researchers in 200 countries through Merck’s global distribution platform.

Applications

2D/3D Culture

Wound Healing

Anti-Aging

HumaTein™ Matrix is an ideal coating material for feeder layer free induced pluripotent stem cell (iPSC) culture. Xeno free and human cell derived Huma Tein is optimized for organoid research.

  • Cancer Research
  • Stem Cell Research
  • Drug Discovery
  • Tissue Engineering & Regenerative Medicine
Characteristics

Native whole ECM

Manufacturing processes do not include harsh chemical processing. HumaTein™ preserves whole ECM components.

Organ specific

With ROKIT’s cell sheet technologies, HumaTein™ provides organ specific stiffness controlled environment.

Regeneration

HumaTein™ is applicable for regeneration studies.

In vivo like
environment

Human primary cell derived HumaTein™ provides the most in-vivo like environment to cells, and this enables translational studies.

Custom ECM

With your own cell choice, you can order customized HumaTein™ Special Matrix.

Easy Control

HumaTein™ is not sensitive to temperature changes. It's users friendly texture type.
about HumaTein™
How to use?
Humatein™ Mixture
Humatein™ Hydrogel
Why Humatein?
  • 99% Preserved Human Cytokine
  • No Harsh Chemical Processing
  • Immune Tolerance + Stiffness In Vivo
  • With Your Own Cell Customizing
Applicable Fields
about HumaTein™
How HumaTein™ is made?

HumaTein™ is Isolated from specific native stiffness matching micro-environment grown human cells. HumaTein™ composition is optimized for a target cell type growth and proliferation in a native-like environment.

about HumaTein™
Humatein Composition
Humatein Growth Factor
GF Type
Average GF Concentration
EGF
0.014ng/㎖
bFGF
0.164ng/㎖
NGF
0.188ng/㎖
PDGFA
2.291ng/㎖
PDGFB
3.595ng/㎖
PDGFC
0.622ng/㎖
PDGFD
1.844ng/㎖
IGF-1
4.431ng/㎖
TGF-β
0.166ng/㎖
VEGFA
0.167ng/㎖
VEGFB
0.114ng/㎖
VEGFC
0.249ng/㎖
VEGFD
0.602ng/㎖
humatein-graph
  • Col6: muscle regeneration
  • Col1: structural and mechanical scaffold
  • Col5, 11: regulatory fibril-forming collagen
  • Col3: structural support to the extracellular space of connective tissues
  • FGG: structural support to the extracellular space of connective tissues
  • EFEMP2: Fibulin 4
  • EMILIN1: Emilin 1
Cytokine ELISA Array Analysis Comparison

Humatein™ is clinically applicable

99%

Contains 506 essential human Cytokines (SMAD9)

Xenogetic ECM is NOT clinically applicable

73%

Lacks about 27% of cytokines Found in human body

In Vivo Tissue Generation
in-vivo-tissue-generation

Masson’s trichrome staining images of the cross-sections of
(A)polycaprolactone (PCL)/collagen/fibrin (CF) and the (B)PCL/CF/Humatein MSC

Result

These results indicated that the PCL/CF/Humatein MSC group formed more new fibrous tissue than the other groups did. In addition, new bony areas in the middle of the fibrous tissues were observed in the PCL/CF/Humatein MSC group.

3D Models
Result

The Salivary organoid cultured by humatein MSC the more Gene expression markers for Acinar cells, Luminar cells than Matrigel and it is similar to the tissues in the mouse body.

Colon organoid using Humatein™ (0.2%)

Actin
Ki-67
DAPI

· Colon organoid with HumaTein™ Essential Matrix (1㎎/㎖)
· Similar size and structure to xenogetic source colon organoid

HumaTein™ Lines
HumaTein Lines
HumaTein Essential Matrix 50mg/5ml
HumaTein Essential Matrix 100mg/100ml
HumaTein MSC Matrix 30mg/3ml
HumaTein MSC Matrix 60mg/6ml
HumaGel Essential 5ml, 10mg/ml
HumaGel Essential 10ml, 10mg/ml
HumaGel MSC 3ml, 10mg/ml
HumaGel MSC 6ml, 10mg/ml
Catalogue#
HT-MD-001-A
HT-MD-001-B
HT-MD-12
HT-MD-13
HT-MDG-01
HT-MDG-02
HT-MDG-03
HT-MDG-04
Culture
Endothelial Cell, Dermal Fibroblast Cell
Stem Cell
Skin, Blood vessle Organoid
Organoid
Materials
Human Primary Neonatal Dermal fibrolast cell
Human Primary Bone marrow stem cell
Humatein Essential + Atelocollagen
Humatein MSC + Atelocollagen
Application
2D Thin Coating, Injection,
Suspension Culture
Thick layer Culture, Dom Culture

※ HumaTein™ is provided in mixture and hydrogel types.

Review

Human mesenchymal stem cell derived extracellular matrix enhances formation and maturation of salivary gland organdies

Yeo Jun Yoon, Ph.D.

Division of Head and Neck, Department of Otorhinolaryngology,
Yonsei University College of Medicine, Seoul

Immediately implantable extracellular matrix-enriched osteoinductive hydrogel-laden 3D-printed scaffold for promoting vascularized bone regeneration in vivo

Materials & Design ( Impact factor : 9.417) 2022-07 | Journal article

JaeSeo Lee, Haram Nah, DongNyoung Heo

Department of Dental Materials, School of Dentistry, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea

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