ECM (Extracellular Matrix) is a protein complex, consisting of more than 300 proteins, peptides, and polysaccharides. ECM supports cell attachment, proliferation and migration, and it roles as a growth factor reservoir.
A cell culture biomaterial supplement consisting
of full native human ECM (Extracellular Matrix),
useful for in vitro 4D bioprinting
and cell culturing as well as clinical translational studies
HumaTein™ is a human cell derived whole Extracellular Matrix(ECM), comprised of more than 300 proteins and associated cytokines.
HumaTein™ opens new possibilities for regeneration researches and relative studies.
ROKIT products are available to researchers in 200 countries through Merck’s global distribution platform.
2D/3D Culture
Wound Healing
Anti-Aging
HumaTein™ Matrix is an ideal coating material for feeder layer free induced pluripotent stem cell (iPSC) culture. Xeno free and human cell derived Huma Tein is optimized for organoid research.
- Cancer Research
- Stem Cell Research
- Drug Discovery
- Tissue Engineering & Regenerative Medicine
Native whole ECM
Organ specific
Regeneration
In vivo like
environment
Custom ECM
Easy Control
- 99% Preserved Human Cytokine
- No Harsh Chemical Processing
- Immune Tolerance + Stiffness In Vivo
- With Your Own Cell Customizing
HumaTein™ is Isolated from specific native stiffness matching micro-environment grown human cells. HumaTein™ composition is optimized for a target cell type growth and proliferation in a native-like environment.
- Col6: muscle regeneration
- Col1: structural and mechanical scaffold
- Col5, 11: regulatory fibril-forming collagen
- Col3: structural support to the extracellular space of connective tissues
- FGG: structural support to the extracellular space of connective tissues
- EFEMP2: Fibulin 4
- EMILIN1: Emilin 1
Humatein™ is clinically applicable
99%
Contains 506 essential human Cytokines (SMAD9)
Xenogetic ECM is NOT clinically applicable
73%
Lacks about 27% of cytokines Found in human body
Masson’s trichrome staining images of the cross-sections of
(A)polycaprolactone (PCL)/collagen/fibrin (CF) and the (B)PCL/CF/Humatein MSC
These results indicated that the PCL/CF/Humatein MSC group formed more new fibrous tissue than the other groups did. In addition, new bony areas in the middle of the fibrous tissues were observed in the PCL/CF/Humatein MSC group.
The Salivary organoid cultured by humatein MSC the more Gene expression markers for Acinar cells, Luminar cells than Matrigel and it is similar to the tissues in the mouse body.
Colon organoid using Humatein™ (0.2%)
· Colon organoid with HumaTein™ Essential Matrix (1㎎/㎖)
· Similar size and structure to xenogetic source colon organoid
Suspension Culture
※ HumaTein™ is provided in mixture and hydrogel types.
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