Proliferation of HDFs on gelatin based three-dimensional scaffolds by 3D bioprinting
Dong Jin Choi (1,2), Sang Jun Park (1), Bon Kang Gu, Seok Chung (2), Chun-Ho Kim (1,*)
Full replacement, restoration, or, regeneration of defective or injured functional living, tissues is important goal in tissue engineering. In order to, achieve these goal, 3D shape of the biomedical scaffold, should be highly porous and with an appropriate pore, size, pore interconnectivity, and exhibit a high surface, area-to-volume ratio1, . In particular, uncontrollable pore, size and porosity may obstruct successful tissue, regeneration. 3D bioprinting technologies is appropriate, method to fabricate these 3D scaffold. Natural, biopolymers, such as gelatin, chitosan and alginate, have, been widely used as 3D bioprinting2-3, . In this study, we, fabricate pore size controlled 3D gelatin scaffolds by, using 3D bioprinting and to evaluate their biological, properties. The pore sizes of 3D scaffolds were controlled, in the range of 600 to 1,200 μm. We successfully, fabricated 3D gelatin scaffold with various pore size by, using 3D bioprinting system with a cryogenic plate. To, evaluate the feasibility of this structure as substrates for, scaffold, human dermal fibroblast (HDFs) were cultured, on the scaffold and the cellular response was compared, with that from various mean pore sizes (600, 800, 1,000,, 1,200 μm) of the scaffold.