FAQ

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What are the areas where INIVIVO is currently being used (or the research of researchers who are scheduled to purchase)?

First of all, we are very interested in the field of basic research on tissue regeneration or transplantation by creating scaffolds such as skin cells/bone cells/ cartilage cells, and in the field of ENT and veterinary medicine, we also use the printouts to print out the shape of the...

What are the strengths of INVIVO as a bio printer other than the output part?

INVIVO is a machine created by a team of mechanical engineers, software engineers, medical scientists, gene/cell therapists, and a number of organizational engineers and doctors to reflect each other’s needs. So there are some features that are designed to be easy to use in...

What other areas can INVIVO be used besides the scanning output for cell reproduction and tissue differentiation?

The output design of the INVIVO is 100mm X 100mm wide and can make it all real, not just a scaffold. For example, a Korean otolaryngologist from WakeForrest, who bought our printer, is in the final stage of his research to create an ear shape with a texture similar to the actual human...

Is it possible to apply the materials developed by the researcher?

Yes, research is an act of finding new values, and it’s meaningless for researchers to use only the materials and mechanisms that are set. ROKIT Healthcare welcomes researchers to upgrade INVIVO and develop and use their own materials. Please make a more valuable and good bio material...

Can one output be combined by using two types of nozzle (extruder and syringe dispenser) at the same time?

Yes, it’s possible. When you create two Stl files and designate them as left and right nozzles in the ROKIT INVIVO software, the INVIVO creates a complex three-dimensional file that fits the g-code it entered. For example, you can use PLGA to create a rigid frame with the outer rim and...

What materials can be printed with right syringe dispenser?

The syringe dispenser may print collagen, gelatin, hyaluronic acid, silk, etc. that can make soft tissue scaffold or structures, and may require additional options due to different material curing methods. Soft, viscous materials also have a high pressure of 6 bar, allowing the...

Can you make a scapold out of biodegradable polymer material for light tissue?

Instead of melting pellet-type polymeric materials, ROKIT Healthcare’s bio 3D printer INVIVO directly develops, produces, and supplies PLGA, PLLA, and PCL filaments, which are bio-medical filaments produced at GMP facilities (manufacturing licensing facilities such as medical devices...

Can I use INVIVO on a clean bench?

yes, depending on the size of the bench you are using and weight you can support, you can remove the door or the HEPA filter then use it.

When using two dispensers, can one dispenser have a certain amount of cell seeding in the scaffold using bio-ink with cells?

Using 3D modeling tools, you can create a single scapold design stl file and a cell seeding file in the desired position and combine it into a single file in Creator K to create a dual print for that purpose.

Can I use only the dispenser syringe that is provided from the ROKIT Healthcare?

The standard mounting system recommends using the provided syringe. However, we are considering a plan to diversify the types of mounting depending on demand so that we can make the most of the existing syringes that are frequently used.

How long would it normally be enough to turn on the UV lamp to sterilize it?

If you leave it on for 30 minutes, it can be sterilized enough, so please avoid leaving it on for too long.

What kinds of bio-materials are you planning to sell in the future?

We plan to increase the lineup of materials that can be useful in bio-organization experiments. We are looking for partners to commercialize specific materials together, so we are always welcome your contact.

How do you store and use INVIVO Gel?

For long-term storage, it is recommended to store in a 4-degree refrigeration environment. When using, make the material completely liquid at 37 degrees C according to the manual, mix it with Cross linker solution at a ration 4:1, and gelling it in a 4 degrees C then you can print with...

In the case of INVIVO Gel, are there any results that have been verified with the actual cells?

Various tests have validated INVIVO Gel’s suitability with cells and photosynthesis to enhance mechanical properties to prevent it from collapsing in a moist environment. (Refer to PPT report provided by salesperson)

What is the storage method or period of use for bio-filament?

Basically, I recommend keeping the seal at room temperature where there is no moisture. Shelf life is about 6 months, but we recommend using it right after purchase.

Is it possible to change the wavelength or power of UV LED and make an order?

Theoretically, it is possible to use UV-LED with 405mm wavelength and power more than 3W. However, there may be housing or stability problems, so please consult the detailed specifications through the salesperson of the ROKIT Healthcare or the technical lab.

Is the inside of the printer completely sealed from the outside? How did you shield it? (Concern about pollution from the outside air)

Complete switching and closing systems are implemented using silicon sealing and powerful door magnets for complete sealing from outside.

Is it possible to make an order in addition to the three types of dispensers provided as standard?

It depends on what you request, except for the basic tip or nozzle. Please contact our sales representative or technical research center for more information.

if the temperature inside the printer becomes high, there will be adverse effects on bio-materials (especially those containing cells). Normally, what is the internal air temperature during printing, and where is the displayed temperature sensed?

The internal air temperature is maintained at a maximum of ~28 to 30 degrees Celsius on an one-hour output basis. Operating the HEPA filter may lower the temperature, and the temperature sensor is attached to the bottom of the dispenser kit to better reflect the temperature of the...

The extruder nozzle heats up the polymer and outputs it. Does the heat affect the biomaterials?

If the material is ejected at 70 degrees Celsius, such as PCL, in addition to the bio-material for the dispenser, there is no heat damage. However, if dual output is intended with biomaterials foe dispensers, polymers such as PLGA and PLLA with high melting points may have heat-induced...

When printing the first line from the dispenser tip or the extruder nozzle at the start of the output, it seems to be difficult to print a line of constant thickness from the beginning due to thin or high volume. How do I solve it?

Once you start the output, you can print the surrounding area once through the skirt menu before printing the main line of the uploaded g-code file. At this point, you will print enough preliminary drawing to output the main width evenly.

During dual printing with standard dispenser, doesn’t bio-materials flow down while the output is stopped and waiting?

On the dispenser, the step motor determines the amount and direction of discharge of the material. The step motor is equipped with a retract function that pulls the dispenser piston in the opposite direction when the dispenser has to stop output, minimizing material loss. Depending on...

What can I do with the software that the Rokit provides? Can I print with stl file?

stl file can be downloaded from the Internet or prepared using various 3D modeling freeware or 3D modeling paid software. INVIVO software allows secondary settings such as the ouput speed, temperature, fill percentage, and fill feature of prepared stl files to optimize the output to...

Is there a problem for your eyes to look at the UV LED?

It’s very bad for people to look at UV LEDs directly through open or transparent doors, unless they’re in a color-bladed environment like the color doors installed on the INVIVO. Therefore, equipment that uses UV LEDs must be closed. INVIVO firstly blocks UV LED light by the color...

What are the specifications of the sterile UV lamp? Is it possible enough for sterilization?

Yes, the wavelengths optimized for ultraviolet sterilization are 250 to 260nm. The UV lamps in the INVIVO have a wavelength of 254nm and the output value is 12W (6W each). The specifications were determined by considering the output value of the UV lamp used in the international...

What are the specifications for UV LED?

Wavelengths are 365nm and total power is 6W (3W x 2ea). The actual energy reached by considering the distance to the nozzle tip and the energy efficiency of the UV LED is about 40mW (20mW x 2ea).

What is the maximum viscosity of the material that can be pushed by a standard dispenser?

allows sufficient discharge to the level of normal toothpaste, and pressure is up to 6 bar. Obviously, smaller diameter of dispenser tip has more disadvantageous to print the high viscosity material. Therefore, you can use it freely if you set the best settings for the materials you use.

Is it possible to use consumer-developed bio material?

Yes, you can use any filament-type or dispenser material.

Is the ouput bed only available with round petri dish?

No, you cannot only use petri dish which is the default bed, but also available to use other types of beds such as glass plate, 6 well plate, etc.

What is the stable output speed?

If you set the output speed within the range of 3 to 10mm/sec on both sides, you can reliably obtain the desired output.

What is the precision of the nozzle?

The dispenser tip provided standard with an inner diameter of 0.1mm to 0.4mm, and the line width accuracy drawn during printing is highly dependent on the viscosity and physical properties of the material being printed.

How can I get the leaflet?

If you apply by email, we will send it to you in a short time.