A cell culture biomaterial supplement consisting
of full native human ECM (Extracellular Matrix),
useful for in vitro 4D bioprinting
and cell culturing as well as clinical translational studies

about ECM
What is ECM?

ECM (Extracellular Matrix) is a protein complex, consisting of more than 300 proteins, peptides, and polysaccharides. ECM supports cell attachment, proliferation and migration, and it roles as a growth factor reservoir.

about HumaTein™
What is HumaTein™?

HumaTein™ is a human cell derived whole Extracellular Matrix(ECM), comprised of more than 300 proteins and associated cytokines.
HumaTein™ opens new possibilities for regeneration researches and relative studies.


2D/3D Culture

Wound Healing


HumaTein™ Matrix is an ideal coating material for feeder layer free induced pluripotent stem cell (iPSC) culture. Xeno free and human cell derived Huma Tein is optimized for organoid research.

  • Cancer Research
  • Stem Cell Research
  • Drug Discovery
  • Tissue Engineering & Regenerative Medicine

Native whole ECM

Manufacturing processes do not include harsh chemical processing. HumaTein™ preserves whole ECM components.

Organ specific

With ROKIT’s cell sheet technologies, HumaTein™ provides organ specific stiffness controlled environment.


HumaTein™ is applicable for regeneration studies.

In vivo like

Human primary cell derived HumaTein™ provides the most in-vivo like environment to cells, and this enables translational studies.

Custom ECM

With your own cell choice, you can order customized HumaTein™ Special Matrix.

Easy Control

HumaTein™ is not sensitive to temperature changes. It's users friendly texture type.
about HumaTein™
How to use?
Humatein™ Mixture
Humatein™ Hydrogel
Fibroblast Mesenchymal Stem Cells
(primary cells)
Patient biopsy derived Cells Your Lab Choice Cells
Humatein™ Essential
Humatein™ MSC
Humatein™ Special Matrix
Cell Culture Process
iPSC Culture
What ROKIT Provides
What ROKIT Supports
about HumaTein™
How HumaTein™ is made?

HumaTein™ is Isolated from specific native stiffness matching micro-environment grown human cells. HumaTein™ composition is optimized for a target cell type growth and proliferation in a native-like environment.

Cytokine ELISA Array Analysis Comparison

Humatein™ is clinically applicable


Contains 506 essential human Cytokines (SMAD9)

Xenogetic ECM is NOT clinically applicable


Lacks about 27% of cytokines Found in human body

3D Models
Humatein MSC Matrix

A) Phase contrast images of iPSC spheroid.
iPSCs are cultured with MSC-derived HumaTein for a day.

B) Analysis of iPSC spheroid size

Colon organoid using Humatein™ (0.2%)


· Colon organoid with HumaTein™ Essential Matrix (1㎎/㎖)
· Similar size and structure to xenogetic source colon organoid

HumaTein™ Lines

HumaTein™️ Lines (lyophilized powder block)

HumaTein™ Essential Matrix 50mg

HumaTein™ Essential Matrix 100mg

HumaTein™ MSC Matrix 30mg

HumaTein™ MSC Matrix 60mg

HumaTein™ Dermal Papillar Matrix 30mg

HumaTein™ Dermal Papillar Matrix 60mg

HumaTein™ Custom Matrix 30mg









※ HumaTein™ is provided in mixture and hydrogel types.


Human mesenchymal stem cell derived extracellular matrix enhances formation and maturation of salivary gland organdies

Yeo Jun Yoon, Ph.D.

Division of Head and Neck, Department of Otorhinolaryngology,
Yonsei University College of Medicine, Seoul


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