February 14, 2020 by admin 0 Comments

Photocurable chitosan as bioink for cellularized therapies towards personalized scaffold architecture

Authors
Chiara Tonda-Turo (a) (b), Irene Carmagnola (a) (b), Annalisa Chiappone (b) (c), Zhaoxuan Feng (d), Gianluca Ciardelli (a) (b), Minna Hakkarainen (d), Marco Sangermano (b) (c)
Abstract
Recent progresses in tissue engineering are directed towards the development of technologies able to provide personalized scaffolds recreating the defect shape in a patient specific manner. To achieve this ambitious goal, 3D bioprinting can be combined with a suitable bioink, able to create a physiological milieu for cell growth. In this work, a novel chitosan-based hydrogel was developed combining photocrosslinking and thermo-sensitive properties. Commercial chitosan (CS) was first methacrylated and then mixed with β-glycerol phosphate salt (β-GP) to impart a thermally induced phase transition. The absence of cytotoxic degradation products and the excellent biocompatibility of the developed hydrogel was confirmed through in vitro tests using different cell lines (NIH/3T3, Saos-2, SH-SY5Y). Cellularized 3D structures were obtained though 3D bioprinting technologies confirming the processability of the developed hydrogels and its unique biological properties.

June 1, 2018 by admin 0 Comments

The Arrival of Commercial Bioprinters – Towards 3D Bioprinting Revolution!

Authors
Deepak Choudhury*, Shivesh Anand, May Win Naing*
Abstract
The dawn of commercial bioprinting is rapidly advancing the tissue engineering field. In the past few years, new bioprinting approaches as well as novel bioinks formulations have emerged, enabling biological research groups to demonstrate the use of such technology to fabricate functional and relevant tissue models. In recent years, several companies have launched bioprinters pushing for early adoption and democratisation of bioprinting. This article reviews the progress in commercial bioprinting since the inception, with a particular focus on the comparison of different available printing technologies and important features of the individual technologies as well as various existing applications. Various challenges and potential design considerations for next generations of bioprinters are also discussed.